According to HitFix, Lee will pop up in the seventh episode of the spy comedy's fifth and final season.
"To say we're excited to have Stan Lee join us on the show is an understatement," Chris Fedak, 'Chuck' co-creator,' told HitFix.
Other guest stars this season include 'Community' star Danny Pudi, Carrie-Anne Moss and Mark Hamill.
Lee has made a number of guest appearances on geek-targeted shows, most recently The CW's 'Nikita.'
In other casting news ...
'Big C' star John Benjamin Hickey will meet a 'A Gifted Man.' Hickey will play Ben, a limo driver with a big secret. Look for him in November. [TVLine]
'New Girl' is adding some new girls of its own.Former 'Saturday Night Live' cast member Michaela Watkins and 'Californication' actress Eva Amurri will guest star in an episode set to air at the end of November. Look for the ladies to cause trouble in Schmidt's (Max Greenfield) professional life. [TV Guide]
Debi Mazar will guest star on 'Melissa & Joey.'Mazar will play Harper Quinn, a political consultant who works with Mel (Melissa Joan Hart) to help smooth over a public scuffle. [TVLine]
'Hart of Dixie' is giving Lemon a younger sister. Young singer Claudia Lee will play Magnolia, the sister to Jaime King's character. [THR]
Nicole Sullivan is Courteney Cox's shrink ... on 'Cougar Town.' Cox's character Jules and her beau Grayson will start seeing Sullivan's potentially recurring character after some issues arise regarding kids. [TVLine]